We will introduce you to the minimum parameters to consider for the proper definition of a chromatographic method and for establishing its controls. The concept of Instrumental Calibration will be reviewed in its different approaches (curve fitting with multiple points, use of the response factor, with and without internal standard, with and without matrix) along with the criteria for its evaluation.
We will explain the validation parameters (linearity, trueness, and precision) and the estimation of the uncertainty of the results.
