COURSE PROGRAM
1. Applications of the PCR Technique
2. Detection of Microorganisms
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- Detection of Genetically Modified Organisms
- Species identification
3. Forensic Genetics (Individual / kinship identification)
4. Introduction to the PCR Technique
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- Phases of the test
- Objectives and characteristics
5. Applicable Regulations
6. ISO/IEC 17025 Requirements Applied to PCR Techniques
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- Facilities and conditions
- Test methods
- Characteristics depending on the field and application
- Event characteristics
- Contents of the methods
- Method validation
- Types of methods and parameters
- Methodology for identification methods
- Methodology for qualitative methods
- Methodology for quantitative methods
- Equipment
- Equipment used
- Operations to be carried out and criteria
- Reference materials and control samples
- Control of reagents: mastermix, probes, primers
- Quantification using Real-Time PCR
- Concept of calibration curve
- Standards, adjustment and acceptance criteria in PCR
- Quality Control
- Activities to be carried out according to phases
- Acceptance criteria
- Application of a plan depending on the type of methods
- Records
- Minimum required contents
- Reports
- Characteristics of result expression
