Instrumental Technique: Chromatography

Name: Instrumental Technique: Chromatography
SKU: 28180
Availability: InStock

Price range: 330,00 € through 570,00 €

We will introduce you to the minimum parameters to consider for the proper definition of a chromatographic method and for establishing its controls. The concept of Instrumental Calibration will be reviewed in its different approaches (curve fitting with multiple points, use of the response factor, with and without internal standard, with and without matrix) along with the criteria for its evaluation.

We will explain the validation parameters (linearity, trueness, and precision) and the estimation of the uncertainty of the results.

Mode

E-Learning
In-person - Streaming

Date

Clear

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Product price:	330,00 €
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Category: Scheduled Courses

COURSE PROGRAM

Chromatographic Methods: Parameter Definition

Mobile Phase: composition and flow
Injection (type and volume)
Columns and ovens
Detector
Indirect control: retention times, response
Identification, spectra, and ratios
General considerations

Instrumental Calibration (types, requirements, and control)

External standard calibration
Internal standard calibration
Linear calibration
Calibration using response factor
Matrix calibration

Reference Materials: Characteristics and Uses
Quality Control and Ongoing Validation

COURSE PROGRAM

INTRODUCTION

1. Concept of Chromatography
2. Types of Chromatographic Separations
3. Basic Concepts in Chromatography
4. Chromatographic System
LIQUID CHROMATOGRAPHY
1. Introduction
2. Classification Based on Stationary Phase
3. Components
4. Most Common Problems
5. Differences Between HPLC and UPLC
6. HPLC Method vs UPLC Method
DEVELOPMENT OF A LIQUID CHROMATOGRAPHY METHOD
1. Sample Processing
2. Mobile Phase Preparation
3. Equipment and Column Conditioning
4. Sample Injection
5. Concentration Gradients
GAS CHROMATOGRAPHY
1. Introduction
2. Chromatographic System
3. Columns
4. Carrier Gas (Mobile Phase)
5. Injectors
6. Temperature Control (Oven)
7. Detectors
8. Development of a Gas Chromatography Method
MASS SPECTROMETRY COUPLED TO CHROMATOGRAPHY
1. Introduction
2. Basic Principles and Technique Operation
3. Most Common Analyzers
4. Tandem Mass Spectrometry
5. Interpretation of Mass Spectrometer Data
CALIBRATION OF CHROMATOGRAPHIC SYSTEMS
1. Instrumental Calibration
2. Calibration Curve
3. Criteria for Calibration Curve Evaluation
4. Complementary Techniques
CHROMATOGRAPHIC TEST METHODS
1. Definition
2. Working Range
3. Materials and Methods
4. Operations